Coding

Part:BBa_K3081122

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)


dCas9-linker-sfGFP-ssrA

This part is an improvement of dCas9-linker-sfGFP(BBa_K3081119), in which we add a degradation signal peptide ssrA to sfGFP.In this part,a fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4147


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Parameters
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